A Shellfish Monitoring System for Paralytic Shellfish Toxins Using Enzyme-Linked Immunosorbent Assay
نویسندگان
چکیده
منابع مشابه
Immunoassay methods for paralytic shellfish poisoning toxins.
The current status of immunochemical techniques for analysis of paralytic shellfish poisoning (PSP) toxins is summarized. Important aspects regarding production of the biological reagents necessary for immunochemical methods, the characteristics of polyclonal and monoclonal antibodies against saxitoxin and neosaxitoxin, and the importance of test sensitivity and specificity are discussed. Appli...
متن کاملParalytic Shellfish Poisoning Toxins: Biochemistry and Origin
Plankton feeders such as bivalves often become toxic. Human consumption of the toxic bivalve causes severe food poisoning, including paralytic shellfish poisoning (PSP) which is the most dangerous because of the acuteness of the symptoms, high fatality and wide distribution throughout the world. Accumulation of PSP toxins in shellfish has posed serious problems to public health and fisheries in...
متن کاملDetermination of okadaic acid in shellfish by using a novel chemiluminescent enzyme-linked immunosorbent assay method.
A direct competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) was developed to determine okadaic acid (OA). Concentrations of the capture monoclonal anti-OA antibodies, conjugate of OA-HRP and a composition of blocking buffers were varied to optimize the assay condition. The values of IC10, IC50 and working range (IC20-IC80) for CL-ELISA were 0.01, 0.07, and 0.03-0.2 ng/mL,...
متن کاملENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL
An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
متن کاملDevelopment of reference materials for paralytic shellfish poisoning toxins.
A project was undertaken to develop mussel reference materials that were certified for their mass fractions of saxitoxin and decarbamoyl-saxitoxin. Fifteen laboratories from various European countries participated. Three of these had major responsibility for substantial parts of the work and overall coordination of the project. The project involved 4 main activities: (1) procurement and charact...
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ژورنال
عنوان ژورنال: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
سال: 2013
ISSN: 0015-6426,1882-1006
DOI: 10.3358/shokueishi.54.397